생명과학연구소 특별세미나_김원근 교수님(한림대)

일시: 2022년 4월 7일(목) 오후5시

zoom link: https://kangwon-ac-kr.zoom.us/j/96935931595?pwd=V05aenZVc2F6Vlc4SlVuU1RnTGZKZz09

Next Pandemic Preparedness and Responsiveness:

Antivirals and Real-time Tracking of Emerging Zoonotic Viruses

김원근 교수 (한림대학교 의과대학 미생물학교실)

Emerging and re-emerging RNA viruses pose critical public health, economic, societal, and cultural burdens in humans. The recent pandemic of Severe acute respiratory syndrome virus 2 (SARS-CoV-2), the etiological agent of Coronavirus disease-19 (COVID-19), presents urgent needs for effective antiviral therapeutics against the emergence of variants. The next-generation sequencing significantly contributes to the acquisition of viral genome sequences, the identification of etiological agents, and the tracking of transmission chains during the outbreak. In addition, the metagenomic study of virome plays a key role in discovering potential zoonotic viruses in wild animals.

Firstly, a nucleic-acid hydrolyzing single chain variable fragment (scFv), 3D8, conferred to the antiviral activity against a variety of RNA and DNA viruses. The scFv was utilized for the development of a broad-spectrum antiviral against SARS-CoV-2 and multiple pathogenic coronaviruses in vitro. Secondly, orthohantavirus infections cause hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome in humans. The genomic epidemiology of Hantaan orthohantavirus (HTNV) from humans and rodents enables to reveal the putative infection sites of HFRS patients, Republic of Korea (ROK). Active  surveillance was well-established: 1) by recovering whole-genome sequences of HTNV, 2) by tracing the potential infection site of patients with HFRS, and 3) by collecting HTNV-positive rodents. The high-resolution phylogeographic relationships of patient- and rodent-derived orthohantaviruses enabled to identify the infection source by temporally and spatially tracking viral genomes. Thirdly, a total of 78 species and 17 genera of paramyxoviruses are currently classified in multiple natural reservoirs. Here, we discovered genetically distinct novel paramyxoviruses, Paju Apodemus paramyxovirus 1 (PAPV-1) and 2 (PAPV-2) in Apodemus agrarius, ROK. The whole-genome sequences of PAPV-1 and -2 showed distinct phylogenetic lineages of Jeilongvirus despite originating from the same natural host species. Also, we found genetically distinct novel paramyxoviruses, Gamak virus (GAKV) and Daeryong virus (DARV) from in Crocidura lasiura and C. shantungensis, respectively. The whole-genome sequences of GAKV and DARV inferred independent phylogenetic lineages of Henipavirus in Crocidura species. PAPV-1 and GAKV infection elicited type I/III interferons and pro-inflammatory cytokines in human epithelial or endothelial cells.

In conclusion, we highlight the development of universal antivirals, genomic epidemiology, and novel discovery and zoonosis scoring of emerging viruses for the next pandemic preparedness and responsiveness.

학력 및 약력

2005                    성균관대학교 유전공학과 (이학사)

2013                    마운트 시나이 아이칸 의과대학 (Icahn School of Medicine at Mount Sinai, New York, USA)

                 (이학박사-미생물학)

2005-2007           샌디에고 백신연구소 (Vaccine Institute of San Diego), 연구원

2007                    유타대학 의과대학 혈액학교실 (Dep. Of Hematology, School of Medicine, University of Utah), 연구원

2010-2013               펜실베니아대학 수의과대학 병리생물학교실 (Dep. Of Pathobiology, School of Veterinary, University of Pennsylvania),

                            객원연구원

2013-2018           고려대학교 의과대학 미생물학교실, 연구강사

2018-현재            한림대학교 의과대학 미생물학교실, 조교수

주요발표논문 (최근) 10편 내외

1)      Geum-Young Lee*, Won-Keun Kim*, Jin Sun No*, Hayne Cho Park, Yongjin Yi, Seung-Chan Cho, Jingyeong Lee, Seung-Ho Lee, Kyungmin Park, Jongwoo Kim, and Jin-Won Song, Clinical and immunological predictors of hemorrhagic fever with renal syndrome outcome during the early phase, Viruses, 14(3), 595; https://doi.org/10.3390/v14030595 (2022) *Equal contribution

2)      Geum-Young Lee*, Won-Keun Kim*, Seungchan Cho, Kyungmin Park, Jongwoo Kim, Seung-Ho Lee, Jingyeong Lee, Young-Sun Lee, Ji Hoon Kim, Kwan Soo Byun, and Jin-Won Song, Genotyping and Molecular Diagnosis of Hepatitis A Virus in Patients Using Multiplex PCR-Based Next-Generation Sequencing, Microorganisms, 10(1), 100; https://doi.org/10.3390/microorganisms10010100 (2022) *Equal contribution

3)      Seung-Ho Lee, Kijin Kim, Jong Woo Kim, Jin Sun No, Kyungmin Park, Shailesh Budhathoki, Seung Ho Lee, Jingyeong Lee, Seung Hye Cho, Geum-Young Lee, Jusun Hwang, Heung-Chul Kim, Terry A. Klein, Chang-Sub Uhm, Won-Keun Kim*, Jin-Won Song*, Discovery and Genetic Characterization of Novel Paramyxoviruses Related to The Genus Henipavirus in Crocidura species in The Republic of Korea, Viruses, 13(10), 2020; https://doi.org/10.3390/v13102020 (2021) *Co-correspondence

4)      Seungchan Cho*, Won-Keun Kim*, Jin Sun No*, Jaehun Jung, Yongjin Yi, Hyane Cho Park, Seung-Ho Lee, Geum-Young Lee, Kyungmin Park, Jeong-Ah Kim, Jongwoo Kim, Jingyeong Lee, Daesang Lee, Dong Hyun Song, Se Hun Gu, Seong Tae Jeong, Jin-Won Song, Urinary Genome Detection and Tracking of Hantaan Virus from Hemorrhagic Fever with Renal Syndrome Patients Using Multiplex PCR-based Next-generation Sequencing, PLoS Neglected Tropical Diseases, 15(9):e0009707 (2021) *Equal contribution

5)      Seung-Ho Lee, Jin Sun No, Kijin Kim, Shailesh Budhathoki, Kyungmin Park, Geum Young Lee, Seungchan Cho, Bong-Hyun Kim, Seunghee Cho, Jong Woo Kim, Jin Gyeong Lee, Seung Hye Cho, Heung-Chul Kim, Terry A. Klein, Chang-Sub Uhm, Won-Keun Kim*, Jin-Won Song*, Novel Paju Apodemus Paramyxovirus 1 and 2, Harbored by Apodemus agrarius in The Republic of Korea, Virology, 562:40-49 (2021) *Co-correspondence

6)      Kyungmin Park*, Won-Keun Kim*, Seung-Ho Lee, Jongwoo Kim, Jingyeong Lee, Seungchan Cho, Geum-Young Lee, Jin Sun No, Keun Hwa Lee, and Jin-Won Song, A novel genotype of Hantaan Orthohantavirus harbored by Apodemus agrarius chejuensis as a potential etiologic agent of Hemorrhagic Fever with Renal Syndrome in Republic of Korea, PLoS Neglected Tropical Diseases, 15(5):e0009400 (2021) *Equal contribution

7)      Gunsup Lee, Shailesh Budhathoki, Geum-Young Lee, Kwang-ji Oh, Yeon Kyoung Ham, Young-Jun Kim, Ye Rin Lim, Phuong Thi Hoang, Yongjun Lee, Seok-Won Lim, Jun-Mo Kim, Seungchan Cho, Tai-Hyun Kim, Jin-Won Song, Sukchan Lee* and Won-Keun Kim*, Broad-spectrum antiviral activity of 3D8, a nucleic acid-hydrolyzing single chain variable fragment (scFv), targeting SARS-CoV-2 and multiple coronaviruses in vitro, Viruses, 13(4), 650 (2021) *Co-correspondence

8)      Byoung Kwon Park, Jinsoo Kim, Sangkyu Park, Dongbum Kim, Minyoung Kim, Kyeongbin Baek, Joon-Yong Bae, Man-Seong Park, Won-Keun Kim, Younghee Lee, Hyung-Joo Kwon, MERS-CoV and SARS-CoV-2 replication can be inhibited by targeting the interaction between the viral spike protein and the nucleocapsid protein, Theranostics, 11(8):3853-3867 (2021)

9)      Won-Keun Kim, Seungchan Cho, Seung-Ho Lee, Jin Sun No, Geum-Young Lee, Kyungmin Park, Daesang Lee, Seong Tae Jeong and Jin-Won Song, Genomic Epidemiology and Active Surveillance to Investigate Outbreaks of Hantaviruses, Frontiers in Cellular and Infection Microbiology, doi: 10.3389/fcimb.2020.532388 (2021) Review article

10)  Geum-Young Lee*, Won-Keun Kim*, Kyungmin Park*, Seung-Ho Lee, Jusun Hwang, Jin Sun No, Seungchan Cho, Daesang Lee, Dong-Hyun Song, Se Hun Gu, Man-Seong Park, Seung Tae Jung, Young-Su Kim, and Jin-Won Song, Phylogeographic Diversity and Hybrid Zone of Hantaan orthohantavirus Collected in Gangwon Province, Republic of Korea, PLoS Neglected Tropical Diseases, 14(10): e0008714 (2020) *Equal contribution

11)  Seung-Ho Lee*, Won-Keun Kim*, Kyungmin Park, Jin Sun No, Geum Young Lee, Heung-Chul Kim, Terry A. Klein, Mi-Sook Min, Seo-Jin Lee, Jusun Hwang,  Man-Seong Park, and Jin-Won Song, Genetic Diversity and Phylogeography of Jeju Orthohantavirus (Hantaviridae) in the Republic of Korea, Virology, 543:13-19 (2020) *Equal contribution

12)  Won-Keun Kim, Jin Sun No, Daesang Lee, Jaehun Jung, Hayne Park, Yongjin Yi, Jeong-Ah Kim, Seung-Ho Lee, Yujin Kim, Sunhye Park, Seungchan Cho, Geum-Young Lee, Dong Hyun Song, Se Hun Gu, Kkothanahreum Park, Heung-Chul Kim, Michael R. Wiley, Patrick S. G. Chain, Seong Tae Jeong, Terry A. Klein, Gustavo Palacios, and Jin-Won Song, Active Targeted Surveillance to Identify Sites of Emergence of Hantavirus, Clinical Infectious Diseases, 70(3): 464-473 (2020)