생명과학연구소 특별세미나_홍철암 교수님(영남대)

일시: 2022. 3. 29 (화) 오후 3시, 장소: 자연과학대학 4호관 211호

제목: Catalytic hairpin assembly (CHA)-assisted assay for simple and sensitive detection of short complementary DNA (cDNA)

Specific DNA sequences are recognized as a powerful biomarker for clinical diagnostics because they are close related to the development of various disease and infections. Currently, the quantitative real-time polymerase chain reaction (qRT-PCR) is among the most widely used method for specific DNA detections. However, this qRT-PCR technique cannot precisely detect short DNA with less than 30 nucleotides due to low binding affinity of the primers. In addition, it also requires expensive equipment and reagents, time-consuming procedures, and appropriate primer designs. In recent years, the catalytic hairpin assembly (CHA) reactions have been introduced as an alternative method for short DNA detection owing to simple and enzyme-free reactions.

The aim of our study is to develop an enzyme-free, simple, and cost-effective assay for short DNA detection with high sensitivity and specificity by CHA reactions and various materials including quantum dots and capillary tubes. So, I would like to present and discuss our recent for short DNA detection in this seminar.