생명과학연구소 특별세미나 _ 이민호 교수님 (한림대)

제목: New insights into regulation of virulence in pathogenic bacteria- From the perspective of adaptation to environmental changes

일시: 2022년 1월 19일(수) 오후 4시 30분

zoom link: https://kangwon-ac-kr.zoom.us/j/81773606160?pwd=MThEeFp2UVVBWWcyOVIzbzV1MmtTdz09

Bacteria utilize RNA metabolism such as RNA processing and decay and generation divergent ribosomal RNA (rRNA), etc. to rapidly adapt to environmental changes. However, the molecular mechanisms underlying divergent rRNA- and ribonuclease-associated pathogenesis have not yet been characterized.

Here, we provide that ribosomes containing the most variable rRNAs, encoded by the rrnI operon (herein designated as I-ribosomes), direct the preferential translation of a subset of mRNAs in Vibrio vulnificus, enabling the rapid adaptation of bacteria to temperature and nutrient shifts. In addition, we also show strong experimental evidence that the coordinated modulation of endoribonuclease activity constitutes an additional regulatory layer upstream of a complex feed-forward loop controlling global regulatory systems in the Salmonella pathogenicity island 1 (SPI-1) type III secretion system (T3SS).

Thus, our study improves the understanding of the mechanisms through which bacterial pathogens sense the host environment and respond precisely by expressing gene products required for adaptation to that particular niche.

학력 및 약력

2001-2007           B. S. Department of Life Science, Chung-Ang University

2007-2009           M. S. Molecular Cell Biology, Chung-Ang University

2009-2012           Ph. D. Molecular Cell Biology, Chung-Ang University

2013-2016           Post-doctoral fellow/Research fellow, Chung-Ang University

2015-2016           Research fellow, British Columbia University, Vancouver, Canada

2016-2021           Research Professor, Department of Life Science, Chung-Ang University

2021-present      Assistant Professor, Department of Microbiology, College of Medicine, Hallym University

주요발표논문 (최근)

1. Lee J, Shin E, Park J, Lee M*, Lee K*. (2021) Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus. J Microbiol 59(12):1133-1141. (*Co-correspondence)

2. Lee J, Lee M*, Lee K*. (2021) Trans-acting regulators of ribonuclease activity. J Microbiol 59(4):341-359. (*Co-correspondence)

3. Lee M*, Ryu M*, Joo M*, Seo YJ*, Lee J, Kim HM, Shin E, Yeom JH, Kim YH, Bae J, Lee K. (2021) Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression. PLoS Pathog 17(2):e1009263. (*equal contribution)

4. Lee M*, Joo M*, Sim M*, Sim SH*, Kim HL*, Lee J, Ryu M, Yeom JH, Hahn Y, Ha NC, Cho JC, Lee K. (2019) The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli. Sci Rep 21;9(1):17257. (*equal contribution)

5. Song W*, Joo M*, Yeom JH*, Shin E*, Lee M*, Choi HK, Hwang J, Kim YI, Seo R, Lee JE, Moore CJ, Kim YH, Eyun SI, Hahn Y, Bae J, Lee K. (2019) Divergent rRNAs as regulators of gene expression at the ribosome level. Nat Microbiol 4(3):515-526. (*equal contribution)

6. Lee M*, Ahn S*, Lim B, Lee DH, Lee K. (2014) Functional conservation of RNase III-like enzymes: studies on a Vibrio vulnificus ortholog of Escherichia coli RNase III. Curr Microbiol 68(4):413-8. (*equal contribution)