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제 513 회 생명과학연구소 정기 세미나 2017. 3. 23 목요일 오후 5시, 자5 101호 |
Genome-wide screening to find potential pro-longevity gut microbes using Caenorhabditis elegans/Escherichia coli system
권 은수 박사 (한국생명공학연구원)
Gut microbes regulate a variety of host physiology such as metabolism, immunity and gastrointestinal homeostasis. However, studies on their effects on host aging are poorly understood due to their complexity. Here, we used a simple model system consisting of a gut microbe (Escherichia coli) – host (Caenorhabditis elegans) to identify genetic interactions between microbes and host aging.
Using Keio Collection, 3985 E. coli single gene knock out library, we performed genome-wide lifespan screening, with scoring the survival rate of C. elegans feeding on each E. coli single gene mutant. Consequently, we isolated 18 E. coli mutants which extend the lifespan of C. elegans and 35 E. coli mutants to reduce the lifespan of C. elegans by the extent of 20% thresholds. In this study, we showed that worms lived ~30% longer on E. coli licA(lifespan increasing candidateA) mutant which encodes one of central glucose metabolic enzymes. Interestingly, this lifespan extension is completely dependent on C. elegans AAK-2, catalytic α subunit of AMPK (AMP-activated protein kinase). In addition we found that phosphorylation of AAK-2 was increased on lic1 E. coli mutant.
To find molecular mechanisms of inter-species genetic regulation of longevity, these experiments are in progress: metabolomics to find metabolites changed in E. coli mutants, phospho-proteomics to find proteins phosphorylated by AAK-2 in lic1 mutant-dependent manner, and lifespan assays using RNAi of possibly involved signaling pathway. These data will provide molecular mechanisms regarding how gut microbes regulate host aging.
Key words: host aging, gut microbes, C. elegans, E. coli, AAK-2
EDUCATION & PROFESSIONAL EXPERIENCE
1990 ~ 1998 학사 서울대학교 미생물학과
1998 ~ 2000 석사 서울대학교 대학원
2000 ~ 2005 박사 서울대학교 대학원
2005 ~ 2006 서울대학교 분자미생물학 실험실/ 박사후 연구원
2006 ~ 2012 University of Massachusetts Medical School/ 박사후 연구원
2012 ~ 현재 한국생명공학연구원 노화과학연구소 / 선임연구원
