특별세미나_이은진 교수님(고려대)

지난 번 운영위원회의 결과, 올해는 상황을 고려해서 정기세미나는 없이 각 교수님 별로 신청하여 기존의 임시세미나의 형태로 세미나를 진행하기로 했습니다.  

10월 30일 이번 주 금요일 오후 4시에 의생명과학대학 B동 128호에서 고려대학교 이은진 교수님을 모시고 특별세미나를 진행하려고 합니다. 

장소가 좁은 관계로 많은 분들을 모실 수가 없어서 이번에도 온라인과 병행하려고 합니다. 

공개강좌로 개설하였으니 많은 참여 부탁드립니다. 

https://kangwon-ac-kr.zoom.us/j/87979408057

Survival Strategies of Intracellular Pathogens inside Macrophages

이은진 교수 (고려대학교 생명과학부)

Intracellular pathogens share a strategy to survive and replicate within a macrophage phagosome by producing virulence proteins. MgtC is one of such proteins that are conserved in intracellular pathogens including Mycobacterium tuberculosis, Salmonella enterica, Yersinia pestis, Brucella suis, and Burkholderia cenocepacia. Being acquired by horizontal gene transfer, genomic location of the mgtC gene differs in each pathogens, however, the mgtC mutants from above intracellular pathogens have a common phenotype: a severe defect in replicating within a macrophage phagosome, suggesting that the MgtC proteins are required for those intracellular pathogens to survive within an acidified phagosomal space. To understand how the MgtC protein helps each pathogen to achieve successful replication inside macrophages, we carried out biochemical and genetic approaches including an in vivo crosslinking experiment followed by immunoprecipitation and a bacterial two-hybrid analysis to search for binding targets. Several proteins are currently identified and investigated to confirm interaction with the MgtC protein, suggesting that MgtC alters pathogen’s bioenergetics and ion transport to promote pathogenicity.

학력 및 약력

1997         서울대학교 미생물학과 (이학사)

1999         서울대학교 미생물학과 (이학석사)

2003         서울대학교 생명과학부 (이학박사)

2003-2010    미국 Washington University in St. Louis (박사 후 연구원)

2010-2013    미국 Yale University (박사 후 연구원/Research Specialist)

2013-2018    경희대학교 유전공학과 (조교수/부교수)

2018-현재     고려대학교 생명과학부 (부교수)

주요발표논문 (최근)

Lee JH, Yoo JS, Kim Y, Kim JS, Lee EJ*, Roe JH* (2020). The WblC/WhiB7 transcription factor controls intrinsic resistance to translation-targeting antibiotics by altering ribosome composition. mBio Apr 14, 11:e00625-20. (*co-corresponding author)

Choi S, Choi E, Cho YJ, Nam D, Lee J, Lee EJ* (2019) The Salmonella virulence protein MgtC promotes phosphate uptake inside macrophages. Nat Commun 10:3326

Choi E, Nam D, Choi J, Park S, Lee JS, Lee EJ* (2019) Elongation factor P controls translation of the mgtA gene encoding a Mg²⁺ transporter during Salmonella infection. MicrobiologyOpen 8:e00680

Choi E, Han Y, Cho YJ, Nam D, Lee EJ* (2017) A trans-acting leader RNA from a Salmonella virulence gene. Proc Natl Acad Sci USA 114:10232-10237.

Choi E, Choi S, Nam D, Park S, Han Y, Lee JS, Lee EJ* (2017) Elongation factor P restricts Salmonella’s growth by controlling translation of a Mg²⁺-transporter gene during infection. Sci Rep Feb 9 doi: 10.1038/srep42098.

Nam D, Choi E, Shin D, Lee EJ* (2016) tRNAPro-mediated downregulation of elongation factor P is required for mgtCBR expression during Salmonella infection. Mol Microbiol 102:221-232.