319회 The Role of Soluble Intercellular Adhesion Molecule-1 in Angiogen…

319회

연사 : 고 용 송 , 경희대학교 동서의학대학원 종양연구팀

제목:  The Role of Soluble Intercellular Adhesion Molecule-1 in Angiogenesis and Tumor Growth

Abstract

  Serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) are elevated in a number of pathologic conditions associated with angiogenesis, including tumor growth. Since the increased levels of sICAM-1 suggested that it may be angiogenic, we tested the ability of sICAM-1 to promote angiogenesis and the mechanisms involved. We found that human recombinant sICAM-1 stimulates chemokinetic endothelial cell migration, endothelial cell tube formation on Matrigel, and sprouting of aortic rings without any effect on endothelial cell proliferation. Soluble ICAM-1 also mediates angiogenesis in the chick chorioallantoic membrane assay. It is active in the nanomolar range in most of these assays suggesting that it could function physiologically in vivo. Tumor cells transfected with sense constructs were found to form faster growing tumors than mock transfected cells. Additionally, we found a Mr = 49,000 molecule that binds to sICAM-1 which may be the surface ligand on endothelial cells. The evidence that sICAM-1 has angiogenic activity suggests a possible role linking inflammation and neovascularization. Furthermore, sICAM-1 may enhance tumor growth by promoting  angiogenesis and escape from immunosurveillance.