397회 Microarray Detection of Food-Borne Pathogens Using Specific Probe…

397회  

일시: 2006. 11. 23

연사: 김혜영, 한국미생물생명공학회 영문지 JMB 편집간사

제목: Microarray Detection of Food-Borne Pathogens Using Specific Probes Prepared by Comparative Genomics

Abstract

Food-borne pathogens are a major global healthproblem, and the rapid detection of food-borne pathogens is needed in epidemiology, the food industry, public health maintenance and bioterrorism prevention. To detect these pathogens, specific 70-mer oligonucleotide probes of 11 major food-borne pathogens were prepared by comparative genomics using the Basic Local Alignment Search Tool (BLAST) program between genomic DNA sequences of food-borne pathogens. Among the prepared probes, 10 probes from each food-borne pathogen were selected and a DNA microarray chip named "FBP-1"was constructed with 224 spots of 70-mer oligonucleotides for the detection of 11 food-borne pathogens. Various genomic DNAs of food-borne pathogens and non-pathogens were labeled with Cy3 dye and hybridized with the microarray chip. The constructed microarray chip showed a high specificity with the genomic DNA of each food-borne pathogen. Microarray data of various bacteria were analyzed and clustered. A phylogenetic tree showed a high discriminative power such that each food-borne pathogen was clustered and non-pathogens were discriminated from food-borne pathogens. These results can be applied as a rapid and convenient method to detect and identify food-borne pathogens in the food industry. In addition, this study demonstrated that genome sequence comparison and DNA microarray have a potential application in epidemiologic and taxonomic studies including food safety and biodefense analysis of microbial pathogens.